1. Analysis was done on females with samples "GF_0169_F","GH_0081_M","GH_0087_F","FF_6136_F" removed, so total sample size per group was: 5 FF, 6 GG, 6 HH 6 FG, 5 GF 6 FH, 6 HF 5 GH, 6 HG (overall 51 mouse: 17 inbred, 34 F1s) 2. Files were taken from ftp server ftp://ftp.csbio.unc.edu/cegs_rnaseq_release3/ version of 5/8/2012. 3. Allele specific reads were produced using tag information for snps and indels. For quality control the following settings were chosen: average(base quality of the read)>=30 read map quality>=10 mask to keep specific reads = 0 mask to remove specific reads flag2rm=1796 (based on samtools format specification http://samtools.sourceforge.net/SAM1.pdf seep page 4) read was considered to be coming from a particular allele if majority of the snps+indels (more than half) cam from this allele. 4. Top 10 genes by parent and strain are attached in the following files: FG_parent.csv FH_parent.csv GH_parent.csv FG_strain.csv FH_strain.csv GH_strain.csv first two letters tell which cross was fit, can be either parent of origin or strain effect. for each file the column names are as follows: gene_id - ensemble gene id short_name - short name of a gene chr - which chromosome it it located on (all should be X) start, end - gene positions pval_strain - p-value of a strain effect pval_parent - p-value of parent of origin effect strain_eff - estimate of the strain effect (negative if expression of F is higher than expression of G, if expression of F is higher than expression of H, if expression of G is higher than expression of H ) parent_eff - estimate of the parent of origin effect (negative if maternal, positive if paternal) columns: GH_tot, HG_tot, GG_tot, HH_tot GH_hapG, GH_hapH, HG_hapG, HG_hapH - averages of normalised counts for each appropriate group, first 4 averages for total read counts, then 4 averages for each allele specific read count. 5. The model applied combined total read counts and allele specific counts as in a model as attached in a manuscript http://www.bios.unc.edu/~zhabotyn/code/genes_2012_06_15/RNAseq.zip 6. This model includes estimation of: strain effect (with test), parent of origin effect (with test), adjusted for individual total counts and Xce effect.